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. 2018 Oct 29;128(12):5307–5321. doi: 10.1172/JCI87191

Figure 7. Upregulation of HDR by direct stimulation of RAD51 activity or overexpression of RAD51 fails to rescue dynasore-induced sensitization to chlorambucil in TNBC cells.

Figure 7

(A and B) Dynasore increases chlorambucil sensitivity of the human BRCA1-mutant TNBC cells HCC1937 (A) and SUM149 (B). (B–D) Sensitization to chlorambucil by dynasore is not reversed by overexpression of RAD51-HA in TNBC cell lines SUM149 (B), HCC1806 (C), and MDA-MB-231-BR3 (MDA-BR3) (D). (E) Overexpression of RAD51-HA does not reduce sensitivity to chlorambucil in MDA-MB-231-BR3 cells with DNM2 knockdown. (F) RS-1 does not reduce the sensitizing effect of dynasore in MDA-MB-231-BR3 cells. (A–F) Shown are means ± SDs (ranges) from n ≥ 3 (A) and n ≥ 2 (B–F) MTS experiments. (B–F) See Supplemental Figures 10 and 11 for Western blots showing RAD51 overexpression and for significance analyses. **P < 0.01 by ANOVA.