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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: Mol Cell. 2018 Aug 2;71(4):487–497.e3. doi: 10.1016/j.molcel.2018.06.037

Fig. 5. Rad52-ChIPseq preserves strand-specific Rad52 signal from an irreparable DSB.

Fig. 5

(A) In the DBS model system strain (Fig. 3B) induction of HO nuclease makes an irreparable DSB at the MAT locus, both ends are then resected 5′-3′ leaving the ssDNA Watson (W) strand on one side and the Crick (C) on the other side to be coated by Rad52. (B) Rad52-ChIPseq signal from the MAT locus after HO induction. Signal coming from the W strand is shown in red while C strand signal is in blue. Rad52-ChIPseq correctly preserve the strand-specific information on Rad52 binding around a DSB.