REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal Rad52	D. Bishop lab	N/A
Rabbit polyclonal Rad53	Abeam	ab104232
Rabbit polyclonal H2A phospho serl29	Abeam	abl5083
Mouse monoclonal V5-tag	Thermo fisher	R960–25
HRP goat anti-mouse	Biorad	1706516
HRP goat anti-rabbit	Biorad	1706515
Mouse monoclonal S9.6 DNA/RNA hybrid	S9.6 (ATCC HB-8730)	N/A
Chemicals, Peptides, and Recombinant Proteins
Hydroxyurea	Sigma	H8627
Nocodazole	Sigma	Ml 404
Alpha-factor	Sigma	T6901
Auxin (3-Indoleacetic acid)	Sigma	13750
SYBR Green DNA I	Thermo fisher	S7563
RNase-A	Thermo fisher	EN0531
Proteinase K	Roche	03115828001
AccI	NEB	R0161S
EcoRV	NEB	R3195S
Ncol	NEB	R3193S
Haelll	NEB	R0108S
BsrGI	NEB	R0108S
Critical Commercial Assays
Accel-NGS IS Plus DNA Library Kit (ssDNA ChIP library)	SWIFT Biosciences	10024
Deposited Data
Rad52-ChIPseq	This paper	GSE110575
Experimental Models: Organisms/Strains
Strains list	This work	See Table S1
Oligonucleotides
qPCR oligos	This work	See Table S2
Software and Algorithms
Bowtie2	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml	N/A
MACS	http://liulab.dfci.harvard.edu/MACS/	N/A
SamTools	http://samtools.sourceforge.net/	N/A
BedTools	http://bedtools.readthedocs.io/en/latest/	N/A
FlowJo	https://www.flowjo.com/	N/A