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. 2018 Nov 29;13(11):e0208568. doi: 10.1371/journal.pone.0208568

Fig 7. Rv1460 binds an Fe-S cluster.

Fig 7

(A) UV-visible spectrum of NifS reconstitution reaction (at 1 and 20 hours) containing Rv1460 (50 μM) (black) or without Rv1460 (grey). The absorbance at 2 minutes was used as a blank for the reaction. (B) Near-UV CD spectrum of NifS reconstitution reaction containing Rv1460 (50 μM). Insert shows the near-UV CD spectrum (Ellipticity in millidegrees) of reactions with (solid line) and without Rv1460 (dashed line). (C) UV-visible spectrum of lithium sulphide reconstitution reaction with (black) and without Rv1460 (50 μM) over time. The absorbance at 2 minutes was used as a blank for the reaction. UV-visible spectrum of the reactions after buffer exchange are indicated in Figure K. in S1 file (D) Near-UV CD spectrum of lithium sulphide reconstitution reaction after buffer exchange containing (23 μM) Rv1460. Insert shows the near-UV CD (Ellipticity in millidegrees) of reactions with (solid line) and without Rv1460 (dashed line) after buffer exchange.