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. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795

Fig 1. Phylogenetic tree of ARF GTPases and ARF-like proteins, and the subcellular localization of Arabidopsis ARF GTPases.

Fig 1

(A) Left: Phylogenetic tree of ARFs and ARF-likes from Arabidopsis (At; green), Human (Hs; magenta) and Yeast (Sc; grey). Class I ARFs, ARL1/2/5/8 and ARFRP1 are conserved among eukaryotes, whereas ARF classes A, B and D are plant-specific. Class II and class III ARFs are present in human and yeast but not in plants. Right: List of Arabidopsis ARF and ARL GTPases with their gene identifiers. (B-Y) Subcellular localization by CLSM of YFP-tagged ARF-GTPases of classes I, A and B expressed from the RPS5a promoter (green channel). (B-I) Class-I ARF (ARFA1c; B; F) co-localized partially with TGN-marker VHA-a1-RFP (magenta; C; D) and Golgi-marker Wave22-mCherry (magenta; G; H). Co-localization in two regions of interest (ROI) in line intensity profiles (E, I). (J-Q) Class-A ARF (ARFB1b; J; N) co-localized partially with TGN-marker VHA-a1-RFP (magenta; K; L) and the Golgi-marker Wave22-mCherry (magenta; O; P). Co-localization in two regions of interest (ROI) in line intensity profiles (M, Q). (R-Y) Class-B ARF (R, V) localized at the plasma membrane and in intracellular punctae. ARFB co-localized partially with TGN-marker VHA-a1-RFP (magenta; S; T) but not with Golgi-marker Wave22-mCherry (magenta; W; X). Co-localization in two regions of interest (ROI) in line intensity profiles (U, Y). Scale bar, 10 μm. (Z-B1) Ultrastructural localization of YFP-tagged ARF GTPases ARF1 (Z, A1) and ARFA (B1) with gold-labelled anti-GFP antibodies. Silver-enhanced 1 nm gold (Z), 6 nm gold (A1-B1). Scale bar (Z-B1), 500 nm.