Fig. 4. Quantitative analysis of nucleic acid amplification on the smartphone.
Measured normalized luminance values as a function of reaction time for four initial template copy number (N. gonorrhoeae DNA samples). Quantification is achieved by applying sigmoidal fits to these data (mean ± sd of 3 replicates) and using t1/2 as an amplification parameter for each curve. The analysis is able to detect N. gonorrhoeae DNA down to a copy number of 3.5 copies/rxn (10μL of reaction volume).