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. Author manuscript; available in PMC: 2019 May 21.
Published in final edited form as: Nature. 2018 Nov 21;563(7733):652–656. doi: 10.1038/s41586-018-0743-5

Extended Data Figure 6. The D3-D14-D53 complex formation is mediated by D53-D2 domain.

Extended Data Figure 6.

(a) Pull-down assay using recombinant ASK1-D3, His-D14, and GST-tagged D53-N, D53-D1 or D53-D2 domains. (b-d) Size-exclusion chromatography analyses of the interaction between: full-length GST-D53, D14-GR24 and ASK1-D3 (b), D14-GR24 and either ASK1-D3 or ASK1-D3ΔCTH (c), D14-GR24, D53D2 with ASK1-D3ΔCTH (d). All gels were resolved by SDS-PAGE and analyzed by Western blot using anti-GST and anti-HIS antibodies (as indicated under a) or Coomassie staining (b-d). All experiments (a-d) were repeated independently at least three times.