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. 2018 Nov 29;8:17462. doi: 10.1038/s41598-018-35576-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of pericyte coverage and pericyte-specific gene and protein expression in 26.5–32 week-old Ins2^AKITA and WT littermate controls. (a,b) Representative images of pericyte-specific protein expression in WT and Ins2^AKITA mice, (a) Aminopeptidase N (ANPEP, green) and Vitronectin (VTN, red); (b) Laminin alpha 2 (LAMA2, green) and desmin (DES, red). Endothelium visualized with PECAM1, cyan. n = 2, scale bar 30 μm. (c) The skeletal length of PECAM1 positive capillaries and ANPEP positive pericytes in Ins2^AKITA and WT was measured and plotted as the percentage of the pericyte length over vessel length (n = 6, *p = 0.0125, student’s t test). (d,e) qPCR analysis on isolated brain microvasculature fragments for pericyte-specific gene Pdgfrb (d) and Rgs5 (e). WT controls are set as 1 and Ins2^AKITA results are presented as fold change over WT (n = 5). n.s. = not significant, student’s t test. Data is presented as mean ± SEM.