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. 2018 Nov 29;8:17462. doi: 10.1038/s41598-018-35576-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of microglia in 26.5–32 week-old Ins2^AKITA and WT littermate controls. (a) Representative images of microglia (AIF1, red) in Ins2^AKITA and WT cerebral cortex. Vasculature is stained with anti-PECAM1 antibody. Notice the fewer microglial processes in Ins2^AKITA cerebral cortex when compared to WT, scale bars 10 μm (n = 6). (b) Quantification of the number of AIF1 + microglia per field in WT and Ins2^AKITA cerebral cortex (n = 6), n.s. = not significant, student’s t test. (c) Quantification of the microglial filament length per field (μm) (n = 6), **p = 0.0012, student’s t test.