Table 1.
RNA sequencing of microvascular fragments. Differentially expressed genes in Ins2AKITA (n = 2) when compared to WT (n = 3) littermate controls. In the mouse brain, Gata2, Sema3G, Slc2a1, and Gm694 displayed restricted endothelial expression.
| Gene symbol | FPKM_WT | FPKM_Ins2AKITA | log2(Ins2AKITA/WT) | p_value |
|---|---|---|---|---|
| Pin1 | 9.54 | 21.42 | 1.17 | 5.00E-05 |
| Slc2a1 | 191.65 | 105.16 | −0.87 | 5.00E-05 |
| Akap8l | 66.40 | 35.47 | −0.90 | 1.00E-04 |
| Miat | 57.07 | 28.77 | −0.99 | 1.00E-04 |
| Col6a1 | 30.61 | 15.40 | −0.99 | 1.00E-04 |
| Rtl1 | 36.01 | 18.07 | −0.99 | 5.00E-05 |
| Net1 | 40.09 | 20.02 | −1.00 | 1.00E-04 |
| Snrnp70 | 124.68 | 55.12 | −1.18 | 5.00E-05 |
| Igsf9b | 22.51 | 9.66 | −1.22 | 5.00E-05 |
| A930011O12Rik | 55.76 | 23.81 | −1.23 | 5.00E-05 |
| Col11a1 | 11.91 | 4.92 | −1.28 | 5.00E-05 |
| Sik1 | 16.27 | 6.69 | −1.28 | 5.00E-05 |
| A230050P20Rik | 60.28 | 24.05 | −1.33 | 5.00E-05 |
| Txnip | 55.65 | 21.76 | −1.35 | 1.00E-04 |
| Usp36 | 30.32 | 11.76 | −1.37 | 5.00E-05 |
| Plat | 36.59 | 14.13 | −1.37 | 5.00E-05 |
| Sema3g | 15.04 | 5.75 | −1.39 | 5.00E-05 |
| Galntl2 | 10.79 | 3.47 | −1.64 | 1.00E-04 |
| Gata2 | 19.73 | 6.13 | −1.69 | 5.00E-05 |
| Stxbp2 | 53.89 | 15.51 | −1.80 | 5.00E-05 |
| Apex2 | 16.89 | 3.32 | −2.35 | 1.00E-04 |
| E2f6 | 146.50 | 13.42 | −3.45 | 5.00E-05 |
| Gm694 | 9.75 | 0.00 | 5.00E-05 |
The rest of the genes had a broad expression pattern (FPKM, fragments per kilobase of exon per million reads mapped).