Fig. 5.

The role of the EPYC1 tandem repeats. a Schematic representation of EPYC1 constructs including deletions or additions of the repeat regions. Note, R1* indicates a chimeric repeat containing a short R2 sequence insertion. b All tested constructs are able to demix Rubisco. Three-minute incubation time prior to imaging within the subsequent 2–6 min. Scale bar 15 µm. c EPYC1 proteins with fewer repeats form flatter droplets than variants with higher valency; conditions as in b. Error bars indicate the s.d. (n = 42–50; data points shown in Supplementary Fig. 8d). d Droplets containing the lowest-valency construct recover more rapidly from photobleaching. Sample size n and s.e.m. indicated. Scale bar 2 µm. e Lower-valency (one to two repeats) EPYC1 variants require higher Rubisco concentrations to phase-separate as measured using the droplet sedimentation assay. EPYC1 variants containing three, four, or five repeats behave similarly