Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 23;37(48):6225–6242. doi: 10.1038/s41388-018-0404-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Effects of tyrosine mutation on Polγ activity and autophagy. a, panel a The activity of Polγ in mitochondria following transfection of wild-type and mutant (Y964F) Polγ expression vectors in Polγ-deficient PCRISPR cells. Relative activity of Polγ was measured and expressed as the fold change (bottom panel). The specificity of Polγ activity was validated by comparing with negative control, which contains template−primer mixture and radio-labeled ATP without enzyme. Panel b The ectopic expression of wild-type and mutant Polγ was detected by western blotting using Flag antibody. Endogenous level of Polγ in PCRISPR cells is also shown. b, panel a The Polγ activity in JB6 cells following coexpression of wild-type or mutant (Y964F) Polγ plasmid along with Polγ siRNA. Relative activity of Polγ was measured and expressed as the fold change (bottom panel). Panel b The suppression of Polγ by siRNA and the overexpression of wild-type and mutant Polγ were confirmed by western blotting using Polγ antibody. The relative level of LC3 II formation in JB6 cells following coexpression of wild-type or mutant (Y964F) Polγ plasmid along with Polγ siRNA or control siRNA is shown (bottom panel). The specificity of Polγ activity was validated by comparing with negative control, which contains template−primer mixture and radio-labeled ATP without enzyme. Each data point represents the mean ± SD of three individual samples. Statistical analysis was performed using one-way ANOVA analysis and Bonferroni’s post-test for multiple-group comparisons. Statistical significance is indicated by asterisks: *p < 0.05 and **p < 0.01