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. Author manuscript; available in PMC: 2019 Mar 11.
Published in final edited form as: Lab Invest. 2018 Sep 11;98(12):1575–1587. doi: 10.1038/s41374-018-0117-5

Figure 5. Analysis of the selected senescence-associated genes in ULM cells induced by replication and MK2206 induced senescence.

Figure 5.

(A) Venn diagrams demonstrate the number of unique and overlapping genes between the two groups (P2 vs. P0 in blue, and MK2206 vs. DMSO in yellow). Four genes shared by serial passaging and MK2206 treatment were SGIP1, SLITRK4, FAM108C1 and WIPI1. (B, C) Western blots of WIPI1 and SLITRK4 expression in replication senescent ULM cells (B, confirmed by upregulation of P21) and MK2206 induced senescence (C, confirmed by loss of pAKT). AKT and β-actin were used as loading control. The band density (right) was quantified (n=3) and expressed as the means ± SD. (D) SA-β-gal staining was performed after overexpression of WIPI1 or SLITRK4 in 2D and 3D primary ULM cells. ULM spheroids (n=5) are shown and color intensity was quantified with Image J for WIPI1 and SLITRK4. *p<0.05, **p<0.01, ***p<0.001.