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. 2018 Nov 29;8:17456. doi: 10.1038/s41598-018-35658-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Madagascar mosquito species phylogenetic analysis using the CO1 gene. (a) Maximum Likelihood phylogenetic analysis of one fragment of the CO1 gene⁴¹ showing a tree with the highest log likelihood (−4419.95), comprising 26 nucleotide sequences. There were a total of 669 positions in the final dataset. (b) Maximum Likelihood phylogenetic analysis for selected Culex mosquito species targeting a second CO1 gene fragment⁴³ showing a tree with the highest log likelihood (−2078.87), comprising 22 nucleotide sequences. There were a total of 638 positions in the final dataset. Wolbachia-infected = (W+), Wolbachia-uninfected = (W−), Rift Valley fever virus-infected = (RVFV+).