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. 2018 Nov 29;8:17456. doi: 10.1038/s41598-018-35658-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum Likelihood phylogenetic analysis from concatenation of four MLST loci (coxA, fbpA, ftsZ and gatB). The tree with the highest log likelihood (−7114.64) is shown incorporating 75 nucleotide sequences. There were a total of 1624 positions in the final dataset. Concatenated sequence data from Wolbachia strains downloaded from the MLST database for comparison are shown in teal (mosquito strains) and black (all other host strains), with isolate numbers from the MLST database shown in brackets. Concatenated Ma. uniformis sequence data from Kenya⁶⁰ (Isolate number 500) downloaded from the MLST database is highlighted in teal and denoted with a clear circle, for comparison with the wUnif-Mad strain (blue with filled circle) detected in this study. Other novel resident strains discovered and characterised in our study are highlighted in colour and denoted with filled circles.