Table 2.
Wolbachia and Asaia infection prevalence rates in adult female mosquitoes using three conserved Wolbachia genes (16S rRNA, ftsZ and wsp).
| Mosquito species | Number | Wolbachia gene PCR amplification | Prevalence (%) | Fisher’s exact post hoc P value (Wolbachia vs. Asaia) | |||
|---|---|---|---|---|---|---|---|
| 16S rRNA | ftsZ | wsp | Wolbachia | Asaia | |||
| Aedes albocephalus | 1 | 0 | 0 | 0 | |||
| Aedes circumlateolus | 1 | 0 | 0 | 0 | 100 (20–100) |
||
| Aedomyia furfuria | 2 | 0 | 0 | 0 | 100 (34–100) |
||
| Aedomyia madagascarica^ | 9 | 9 | 9 | 9 |
100
(70–100) |
89
(56–98) |
>0.99 |
| Culex antennatus^ | 32 | 1 | 1 | 0 |
3
(1–16) |
56
(38–74) |
>0.99 |
| Culex bitaeniorhynchus | 12 | 0 | 0 | 0 | 58 (32–81) |
||
| Culex decens^ | 17 | 3 | 3 | 3 |
18
(4–43) |
18
(4–43) |
0.46 |
| Culex duttoni^ | 1 | 1 | 1 | 0 |
100
(21–100) |
100
(21–100) |
>0.99 |
| Culex tritaeniorhynchus^ | 19 | 0 | 0 | 0 | 95 (74–100) |
||
| Culex giganteus | 5 | 0 | 0 | 0 | 80 (37–96) |
||
| Culex pipiens complex | 44 | 0 | 0 | 0 | 7 (1–30) |
||
| Culex poicilipes | 21 | 0 | 0 | 0 | 48 (28–68) |
||
| Ficalbia circumtestacea | 3 | 1 | 1 | 0 |
33
(6–79) |
||
| Mansonia uniformis^ | 24 | 7 | 7 | 0 |
29
(15–49) |
67
(47–82) |
0.65 |
| Uranotaenia spp^ | 27 | 7 | 7 | 7 |
26
(13–45) |
19
(8–37) |
0.54 |
An individual was considered Wolbachia-infected when any one of the three Wolbachia gene fragments were amplified. Species containing resident Wolbachia strains are in bold. Wolbachia and Asaia prevalence rates are shown with 95% confidence intervals in parentheses. Fisher’s exact post hoc test P value is shown comparing Wolbachia and Asaia infections in individual mosquitoes from species that contained at least one individual of both bacterial endosymbionts. ^ denotes where CO1 sequences were obtained for phylogenetic analysis of mosquito species.