Fig. 5.

Disruption of SOX9 impact biological behaviours of CCA cells. a MTT analyses were performed to measure cell viability in different CCA cells with or without SOX9 knockdown. b FACS analyses were used to examine the impact of SOX9 on cell cycle of CC-SW1 and EGI-1 cells. c mRNA and protein expression of EpCAM were measured by qPCR and western blot in CC-SW1 and EGI-1 cells with or without SOX9 knockdown. d Tumour sphere formation assay was used to assess self-renewal of CC-SW-1 cells with or without SXO9 knockdown. e The impact of SOX9 on CC-SW-1 cell migration was examined with transwell migration assay. NS not significant; *, P<0.05; **, P<0.01, ****, P<0.0001