Table 1.
Sequencing information and qPCR data.
| Sample Name | U9 | U8 | U7 | PV4 | PV2 | U6 | P3 | PV6 | P4 | P2 | PV8 | P1 | U11 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rock type | BS | BS | BS | BS | BS | TZ | BS | TZ | GR | TZ | GR | GR | GR |
| No. of reads for analysis | 25947 | 20240 | 211610 | 167934 | 132271 | 97890 | 16707 | 20731 | 15819 | 11146 | 72866 | 13492 | 205810 |
| No. of archeael OTUs | 70 | 9 | 97 | 46 | 98 | 54 | 73 | 66 | 21 | 54 | 49 | 82 | 83 |
| No. of bacterial OTUs | 7545 | 6450 | 28582 | 25949 | 12547 | 21023 | 7544 | 8067 | 6027 | 4987 | 10196 | 6306 | 34080 |
| Unassigned OTUs | 235 | 54 | 877 | 758 | 238 | 698 | 275 | 310 | 192 | 107 | 207 | 155 | 1035 |
| Percentage of reads unassigned | 3.80 | 0.62 | 1.12 | 0.71 | 0.41 | 1.50 | 2.07 | 3.91 | 3.26 | 1.12 | 0.51 | 1.47 | 1.21 |
| % of reads assigned to Archaea | 0.80 | 0.28 | 0.64 | 0.16 | 0.70 | 0.35 | 0.99 | 0.70 | 0.41 | 4.98 | 0.12 | 6.33 | 0.24 |
| % of reads assigned to Bacteria | 95.40 | 99.09 | 98.23 | 99.13 | 98.89 | 98.16 | 96.94 | 95.39 | 96.33 | 93.90 | 99.36 | 92.20 | 98.54 |
| % reads assigned unique OTUs | 38.05 | 12.72 | 19.16 | 15.95 | 4.98 | 17.27 | 35.14 | 32.61 | 29.25 | 22.89 | 5.82 | 23.21 | 18.19 |
| chao1 | 22162 | 16779 | 62595 | 62604 | 34761 | 54564 | 22860 | 23240 | 16919 | 16702 | 28177 | 19284 | 78727 |
| Shannon’s index | 11.22 | 11.18 | 11.13 | 10.82 | 5.02 | 11.78 | 11.88 | 11.91 | 11.51 | 11.14 | 6.47 | 11.54 | 11.51 |
| Simpson’s index | 1.00 | 1.00 | 0.99 | 1.00 | 0.59 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 0.72 | 1.00 | 0.99 |
| Goods coverage | 0.80 | 0.79 | 0.92 | 0.90 | 0.93 | 0.86 | 0.66 | 0.72 | 0.73 | 0.66 | 0.90 | 0.65 | 0.90 |
| Observed OTUs# | 2898 | 2649 | 2487 | 2657 | 3320 | 3172 | 6205 | 3170 | 3995 | 2981 | 2642 | 5097 | 2524 |
| chao1# | 7135 | 6400 | 6666 | 7854 | 9746 | 7889 | 17175 | 7084 | 11758 | 7897 | 6891 | 13613 | 7718 |
| Shannon’s index# | 10.30 | 9.97 | 9.27 | 9.26 | 3.94 | 10.45 | 11.62 | 10.68 | 10.67 | 10.69 | 4.87 | 11.01 | 9.50 |
| Simpson’s index# | 1.00 | 1.00 | 0.99 | 0.99 | 0.52 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 0.62 | 1.00 | 0.98 |
| Estimated bacterial cells | 7.3E + 05 | 4.2E + 05 | 1.8E + 05 | ND | 8.1E + 04 | 1.1E + 05 | 3.2E + 05 | 2.1E + 05 | 5.2E + 05 | 9.6E + 05 | 1.1E + 05 | 2.8E + 05 | 6.8E + 05 |
| Estimated archaeal cells | 1.3E + 03 | 2.8E + 04 | 1.4E + 04 | ND | 5.4E + 03 | 8.0E + 03 | 1.4E + 04 | ND | 1.1E + 02 | 5.5E + 04 | 2.9E + 03 | 5.7E + 02 | 1.8E + 04 |
| dsrB copy number | 6.4E + 04 | 2.7E + 05 | 1.2E + 05 | ND | 5.4E + 04 | 6.6E + 04 | 1.0E + 05 | 7.6E + 02 | 7.2E + 04 | 8.6E + 04 | 5.3E + 04 | 3.1E + 04 | 1.4E + 04 |
| mcrA copy number | 4.1E + 01 | 1.6E + 03 | 9.8E + 02 | ND | 2.1E + 02 | 4.1E + 02 | 2.5E + 03 | ND | ND | 8.5E + 02 | 2.5E + 02 | 3.8E + 02 | 4.8E + 02 |
ND- not detected; all the estimated cell numbers and gene copy numbers are measured per gram of rock. Samples from basalt, transition and granitic zones are designated as BS, TZ and GR, respectively. #Depicts subsampled alpha diversity data.