Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 29;9:5055. doi: 10.1038/s41467-018-07477-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Main chain mutagenesis in a voltage-gated potassium channel voltage-sensor. a Structure of the voltage-sensing domain (VSD) of the Kv1.2/2.1 chimera (pdb 2R9R). The S4-helix is in pink with side-chains for conserved Arg and Lys residues shown as sticks. Right, sequence alignments of the S4 sequence. Residues mutated in Shaker are shown in red. The α-helical (red) and 3₁₀-helical (blue) regions in the Kv1.2/2.1 structure are shown above the alignment. b Structures of amino acids (aa) and α-hydroxy acids (ah): Vah hydroxyl-isovaleric acid, Lah 2-hydroxy-4-methylpentanoic acid, Fah 3-phenyl lactic acid, Iah 2-hydroxy-3-methylpentanoic acid. Right, these are ligated to the tRNAs (pdb 2ZNI) for nonsense suppression. Shaker cRNA containing a UAG stop codon at the suppression site is co-injected with an orthogonal amber (TAG) suppressor tRNA chemically ligated to an amino acid or α-hydroxy acid (red)