Table 1.
Gating parameters with extracellular K+
| V d | z | V d | Z | ||
|---|---|---|---|---|---|
| WT | −25.9 ± 3.4 (10) | 3.4 ± 0.7 (10) | |||
| L358Leu | −25.6 ± 2.3 (7) | 3.2 ± 0.3 (7) | L358Lah | −14.4 ± 3.6 (7) | 3.1 ± 0.6 (7) |
| I360Ile | −22.2 ± 2.6 (7) | 2.7 ± 0.5 (7) | I360Iah | −13.9 ± 4.5 (5) | 4.8 ± 0.9 (5) |
| L361Leu | −28.1 ± 3.0 (8) | 3.2 ± 0.7 (8) | L361Lah | 1.3 ± 3.7 (13) | 3.0 ± 0.4 (13) |
| V363Val | −28.6 ± 3.8 (4) | 3.3 ± 0.2 (4) | V363Vah | −14.7 ± 5.1 (5) | 2.2 ± 0.3 (5) |
| I364Ile | −21.3 ± 0.6 (3) | 2.9 ± 0.3 (3) | I364Iah | −11.2 ± 5.2 (7) | 2.5 ± 0.6 (7) |
| L366Leu | −23.5 ± 2.5 (8) | 2.8 ± 1.0 (8) | L366Lah | −17.8 ± 1.9 (11) | 2.0 ± 0.5 (11) |
| V367Val | −24.0 ± 4.1 (7) | 3.1 ± 0.7 (7) | V367Vah | 1.4 ± 5.3 (8) | 2.3 ± 0.5 (8) |
| V369Vala | −25.5 ± 6.0 (9) | 3.8 ± 0.6 (9) | V369Vaha | 33.0 ± 7.9 (10) | 1.9 ± 0.2 (10) |
| F370Phe | −21.5 ± 2.9 (5) | 2.8 ± 0.3 (5) | F370Fah | 13.6 ± 2.0 (7) | 2.9 ± 0.2 (7) |
| L375Leu | −26.0 ± 2.6 (8) | 3.5 ± 0.6 (8) | L375Lah | −23.4 ± 3.8 (14) | 3.8 ± 0.6 (14) |
Mean values are shown ± standard deviation with n values within parentheses. Tail currents at −50mV were used to determine parameters except for V369 mutations
aIonic current normalized to driving force were used to determine G–Vs for V369Val and V369Vah