Fig. 3.

Expression of GatA enables co-consumption of d-glucose and d-galUA. d-galUA utilization strains expressing no transporter (yRJP064), Gal2p (yRJP054) or GatA (yRJP093) were incubated in pH 3.5 media containing 20 g/L d-galUA (a) as the sole carbon source or b, c d-galUA and d-glucose at high density (OD₆₀₀ = 75). d Hexose transporters are necessary for consumption of d-galUA at low pH and GatA rescues consumption of a hexose knockout strain. Wild-type and hexose knockout (HXT1-7, GAL2) d-galUA utilization strains expressing no transporter (yRJP193, yRJP195) or GatA (yRJP194, yRJP196) were incubated in pH 3.5 media containing 20 g/L d-galUA (OD₆₀₀ = 10). Error bars represent standard deviation of biological triplicates