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Membrane permeabilization assays with E. coli ATCC 25922. (A) NPN uptake assay to check the outer membrane permeabilization. (B) Inner membrane permeabilization using fluorescent dyes SYTO9 and PI; the concentration of each compound is 10 μg/ml. Control in (B) is cells without peptide treatment. Data is plotted as Mean ± SD of three replicates. Two biological repeats were performed.
