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. 2018 Nov 23;9:1356. doi: 10.3389/fphar.2018.01356

FIGURE 1.

FIGURE 1

Wogonin induced cell death via caspase-dependent apoptotic pathway in human CRC cells. (A) Wogonin inhibited human CRC HCT-116 cells viability of in a concentration dependent manner. HCT-116 cells were treated with various concentrations (0–100 μM) of wogonin for 72 h as measured by MTT assay. (B) Effects of wogonin on the colony formation abilities of HCT-116 cells. Colony formation rate was showed in histogram. (C) Effects of wogonin on apoptotic cell death in HCT-116 cells. HCT-116 cells treated with wogonin for 48 h, and the proportion of apoptotic cells was determined by flow cytometry using Annexin V/propidium iodide (PI) double staining. (D) The expression levels of cleaved caspase 3 and 9 in9 in HCT-116 cells after wogonin treatment were measured using Western blot assay. GAPDH was used as an equal loading control. (E) Western blot analysis of autophagy-related proteins including LC3-II and LC3-I in HCT-116 cells after wogonin treatment for 72 h. GAPDH was used as an equal loading control. The results represent the mean of three independent experiments. Values represent mean ± SD, significant difference versus control group, ∗∗∗P < 0.001.