Figure 2.

HIV RNA is more abundant in CD14+CD11c+ dendritic cells than CD14+CD11c– macrophages in infected human cervical explants. (A) HIV infection measured by gag qRT-PCR relative to GAPDH in sorted cells from human cervical explants 20 h post-infection with HIV strain JRCSF or JRCSF packaged with Vpx protein and uninfected control samples. Indicated are mean and SD for five healthy donor tissues (each dot represents data from an individual donor). (B) CD3 expression, measured by qRT-PCR (representative data from one donor) was used to confirm the purity of the sorted populations. qRT-PCR values below the level of detection were arbitrarily assigned the value 0.00001. ^*p < 0.05, ^**p < 0.01, paired t-test.