Figure 3.

p24 Ag+ myeloid cells in human cervical explants analyzed by Imaging Flow Cytometry. Intracellular HIV- p24 Ag staining of a single cell suspension prepared from human cervical explants 20 h post-infection with HIV strain JRCSF. Myeloid cells were identified by cell surface CD14 staining. (A) Shown are representative images of uninfected and infected CD14+ and CD14- cells in one of three donor samples stained for CD14, p24 Ag, and DAPI (BF, brightfield image, scale bar 7 μm). (B) Percent of p24 Ag+ CD14– and CD14+ cells in HIV-infected cervical explants from three donors (each dot represents data from an individual donor). The lines indicate median and range. ^**p < 0.01, paired t-test.