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. 2018 Nov 23;9:2725. doi: 10.3389/fimmu.2018.02725

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Copyright © 2018 Guriec, Bussy, Gouin, Mathiaud, Quero, Le Goff and Collén.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokines mRNA are transcribed in monocytes in response to ulvan. Ulvan was given per os at four different concentrations (0, 10, 25, 50 mg/l) to the animals at day 0. Blood samples (1 ml per animal) were taken from day 0 to day 3. Cellular pellets of three animals were pooled to obtain sufficient number of cells for purification and RT-qPCR. Data represents the mean ± SEM of three independent experiments with 25 chickens per group. Different letters with the same color indicate statistically different values for the different doses at the same time with p < 0.05.