. 2018 Nov 23;9:2725. doi: 10.3389/fimmu.2018.02725

Table 5.

TLR2 and TLR4 pathways regulate transcription in heterophils through common intracytoplasmic mediators.

Inhibitor target	IL1β	IFNα	IFNγ	IL8	IL18	TLR2	TLR4
FOLD CHANGES, HETEROPHILS
No inhibitor	43.53 ± 3.51	21.33 ± 1.405	88.03 ± 1.89	4.31 ± 2.93	3.76 ± 0.36	6.40 ± 0.59	5.14 ± 0.56
TLR2	20.04 ± 2.41	11.76 ± 0.70	45.93 ± 4.42	1.84 ± 0.371	2.15 ± 0.22	2.71 ± 0.26	2.56 ± 0.11
TLR2 + NFKB	7.21 ± 0.54^a	6.18 ± 0.56^a	25.81 ± 1.71^a	1.08 ± 0.11^a	1.03 ± 0.11^a	1.23 ± 0.18^a	1.45 ± 0.15^a
TLR2 + ERK	7.50 ± 0.22^a	7.81 ± 0.65^a	26.49 ± 1.97^a	1.00 ± 0.03^a	0.97 ± 0.10^a	1.97 ± 0.18	3.44 ± 0.27
TLR2 + JNK	3.45 ± 0.27^b	11.42 ± 0.98	41.81 ± 2.63	0.99 ± 0.07^a	0.45 ± 0.03^b	2.39 ± 0.24	3.65 ± 0.21
TLR2 + p38	3.11 ± 0.14^b	10.69 ± 0.82	45.14 ± 5.36	0.91 ± 0.05^a	0.53 ± 0.05^b	2.60 ± 0.30	3.83 ± 0.28
TLR2 + PLC	19.41 ± 1.60	10.43 ± 1.29	42.46 ± 3.42	1.50 ± 0.08	1.87 ± 0.15	2.60 ± 0.23	3.23 ± 0.31
TLR2 + PKC	19.72 ± 0.71	12.19 ± 1.16	43.52 ± 3.91	1.45 ± 0.12	1.78 ± 0.68	2.59 ± 0.27	3.08 ± 0.30
TLR2 + PI3K	16.44 ± 0.44	11.33 ± 0.77	45.16 ± 4.13	1.50 ± 0.16	1.71 ± 0.13	4.04 ± 0.41^a	5.57 ± 0.49^a
TLR4	14.21 ± 0.80	10.46 ± 1.12	48.62 ± 4.17	1.86 ± 0.20	1.74 ± 0.18	2.41 ± 0.19	2.70 ± 0.21
TLR4 + NFKB	5.78 ± 0.47^a	5.34 ± 0.48^a	27.13 ± 2.32^a	0.51 ± 0.03^b	0.71 ± 0.06^a	1.32 ± 0.14^a	1.01 ± 0.10^a
TLR4 + ERK	7.62 ± 0.44^a	6.55 ± 0.72^a	22.36 ± 1.28^a	0.53 ± 0.04^b	0.73 ± 0.06^a	1.85 ± 0.18	2.49 ± 0.22
TLR4 + JNK	3.25 ± 0.31^b	9.86 ± 0.86	41.62 ± 3.89	0.53 ± 0.04^b	0.81 ± 0.08^a	2.29 ± 1.17	2.07 ± 0.21
TLR4 + p38	2.01 ± 0.13^b	9.68 ± 0.86	43.38 ± 4.11	0.61 ± 0.06^b	0.49 ± 0.04^b	2.14 ± 0.22	2.43 ± 0.21
TLR4 + PLC	13.44 ± 0.88	10.24 ± 1.01	44.05 ± 4.29	1.61 ± 0.17	1.75 ± 0.18	1.97 ± 0.20	2.22 ± 0.18
TLR4 + PKC	13.21 ± 0.65	10.43 ± 0.96	46.20 ± 3.96	1.63 ± 0.21	1.55 ± 0.17	2.27 ± 0.20	2.17 ± 0.18
TLR4 + PI3K	13.03 ± 0.76	11.76 ± 1.31	46.71 ± 4.55	1.48 ± 0.12	1.67 ± 0.14	4.43 ± 0.43^a	6.98 ± 0.61^a

Variations are expressed as mRNA fold changes using β-actin as an internal standard. Heterophils (8 × 10⁶/ml) were first incubated for 30 min with the inhibitors before addition of the ulvan extract at 25 μg/ml for 4 h. Data represents the mean ± SEM of four independent experiments in triplicate. Letters indicate statistically significant differences with the experiment with the TLR inhibitor,

p < 0.05,

p < 0.01.