. 2018 Nov 23;9:2725. doi: 10.3389/fimmu.2018.02725

Table 7.

Transcription in monocytes relies on TLR2 and TLR4 activation and requires intra-cellular mediators that differ according to the genes.

Inhibitor target	IL1β	IFNα	IFNγ	IL8	IL13	TLR2	TLR4	iNOS
Fold changes, monocytes
No inhibitor	24.93 ± 2.43	42.96 ± 3.21	64.54 ± 5.01	12.09 ± 1.43	4.25 ± 0.50	12.39 ± 0.93	29.84 ± 2.18	53.59 ± 4.69
TLR2	12.24 ± 1.12^a	15.14 ± 1.53^b	28.89 ± 0.84^a	4.01 ± 0.38^a	2.14 ± 0.12^a	5.41 ± 0.27^a	15.62 ± 1.15^a	27.34 ± 2.23^a
TLR4	10.43 ± 0.97^a	11.79 ± 1.54^b	26.32 ± 0.62^a	5.04 ± 0.55^a	1.78 ± 0.12^a	8.69 ± 0.17^a	11.40 ± 1.01^a	25.58 ± 2.40^a
TLR2+4	1.16 ± 1.28^c	2.08 ± 0.19^c	1.09 ± 0.09^c	0.99 ± 0.08^c	1.21 ± 0.11^a	1.08 ± 0.10^b	1.16 ± 0.11^c	2.89 ± 0.30^c
TLR4+21	4.74 ± 0.35^b	11.79 ± 0.10^b	24.30 ± 0.71^a	5.74 ± 0.32^a	1.88 ± 0.19^a	7.78 ± 0.21^a	12.51 ± 0.14^a	25.87 ± 2.41^a
TLR9	20.13 ± 2.08	39.58 ± 2.85	58.44 ± 4.45	11.95 ± 1.53	4.23 ± 0.07	11.62 ± 0.84	25.09 ± 2.31	55.74 ± 2.30
Cytoplasmic TLR	25.05 ± 3.69	41.88 ± 3.56	68.33 ± 6.04	11.78 ± 1.43	4.33 ± 0.19	12.36 ± 0.86	27.92 ± 2.47	54.58 ± 3.08
NOD	26.57 ± 0.70	43.27 ± 2.27	67.48 ± 6.12	12.45 ± 1.47	4.11 ± 0.08	12.08 ± 0.99	29.67 ± 3.23	50.56 ± 3.95
Dectin	24.49 ± 1.41	44.31 ± 2.36	66.87 ± 5.97	12.35 ± 1.74	4.13 ± 0.32	11.93 ± 1.02	30.55 ± 3.61	54.77 ± 4.38
NLRP1+3	24.73 ± 1.81	42.24 ± 2.27	67.30 ± 6.66	1.81 ± 0.96	4.24 ± 0.15	11.59 ± 0.99	29.67 ± 3.01	54.39 ± 4.70
NLRP3	25.31 ± 1.82	41.91 ± 1.36	64.07 ± 5.85	12.52 ± 1.60	4.48 ± 0.24	12.16 ± 1.05	27.78 ± 2.67	52.99 ± 3.36
NF-KB	3.66 ± 0.32^b	5.96 ± 0.55^c	5.70 ± 0.52^c	2.88 ± 0.23^a	2.49 ± 0.19^a	5.34 ± 0.30^a	4.43 ± 0.36^a	8.92 ± 0.82^c
ERK	6.34 ± 0.59^b	44.86 ± 4.81	44.61 ± 4.22^a	4.26 ± 0.38^a	4.12 ± 0.42	12.38 ± 1.12	32.51 ± 2.85	51.75 ± 3.85
JNK	11.14 ± 1.12^a	44.12 ± 4.34	66.85 ± 5.86	4.17 ± 0.47^a	4.05 ± 0.37	11.72 ± 1.06	30.70 ± 2.72	55.49 ± 3.95
p38 MAPK	4.02 ± 0.35^b	43.36 ± 3.98	64.97 ± 4.79	4.58 ± 0.34^a	4.55 ± 0.31	13.03 ± 1.23	29.95 ± 2.62	57.23 ± 4.21
PLC	14.71 ± 1.13^a	43.87 ± 4.08	68.40 ± 5.95	11.50 ± 1.06	4.23 ± 0.41	12.85 ± 1.25	30.69 ± 2.96	54.75 ± 5.22
PKC	13.1 ± 1.11^a	41.42 ± 4.12	65.92 ± 5.19	11.55 ± 1.14	4.12 ± 0.39	12.69 ± 1.30	31.33 ± 3.12	53.74 ± 5.29
PI3K	13.41 ± 1.24^a	41.15 ± 3.75	65.73 ± 6.23	3.67 ± 0.19^a	4.23 ± 0.43	18.74 ± 1.82^a	57.72 ± 4.16^b	117.73 ± 9.86^c

For the inhibition experiments, monocytes were first incubated for 30 min with inhibitors specific for different PRRs' or intracellular proteins before addition of the ulvan extract at 25 μg/ml for 4 h. Data represents the mean ± SEM of five independent experiments in duplicate. Different letters indicate statistically significant differences, ^ap < 0.05, ^bp < 0.01, ^cp < 0.05.