. 2018 Nov 23;9:2725. doi: 10.3389/fimmu.2018.02725

Table 8.

TLR2 and TLR4 pathways regulate transcription in monocytes through common intracytoplasmic mediators.

Inhibitor target	IL1β	IFNα	IFNγ	IL8	IL13	TLR2	TLR4	iNOS
FOLD CHANGES, MONOCYTES
No inhibitor	25.39 ± 0.66	43.40 ± 2.18	66.39 ± 2.67	11.90 ± 1.09	4.41 ± 0.39	12.22 ± 1.13	29.45 ± 2.28	55.72 ± 4.63
TLR2	12.22 ± 1.58	15.06 ± 1.48	27.39 ± 1.41	4.48 ± 0.23	2.16 ± 0.16	5.98 ± 0.52	15.14 ± 1.48	26.14 ± 2.13
TLR2 + NFKB	1.41 ± 0.24^b	0.88 ± 0.21^b	1.83 ± 0.38^b	1.19 ± 0.21^a	0.90 ± 0.10^a	0.87 ± 0.09^a	1.04 ± 0.11^b	1.03 ± 0.10^b
TLR2 + ERK	3.25 ± 0.31^b	12.97 ± 1.97	9.36 ± 0.77^a	1.43 ± 0.11^a	2.04 ± 0.018	2.63 ± 0.24	13.21 ± 1.19	22.68 ± 2.53
TLR2 + JNK	5.24 ± 0.41^a	13.69 ± 0.96	27.46 ± 2.59	1.78 ± 0.13^a	2.04 ± 0.17	3.14 ± 0.25	15.38 ± 1.49	26.15 ± 2.05
TLR2 + p38	1.87 ± 0.15^b	13.00 ± 1.41	24.93 ± 1.57	1.63 ± 0.14^a	2.00 ± 0.11	3.08 ± 0.29	14.73 ± 1.31	25.53 ± 2.24
TLR2 + PLC	5.66 ± 0.34^a	14.63 ± 2.72	27.80 ± 2.29	4.39 ± 0.46	2.03 ± 0.20	3.24 ± 0.23	14.33 ± 1.07	24.09 ± 2.19
TLR2 + PKC	4.74 ± 0.30^a	12.49 ± 1.18	26.58 ± 2.64	4.49 ± 0.42	2.11 ± 0.18	3.24 ± 0.31	14.91 ± 1.44	25.95 ± 1.99
TLR2 + PI3K	3.19 ± 1.20^b	13.09 ± 1.54	28.13 ± 1.83	2.77 ± 0.10^a	2.14 ± 0.21	10.76 ± 1.03^a	30.82 ± 2.96^a	48.23 ± 4.29^a
TLR4	10.44 ± 1.50	11.123 ± 1.17	21.99 ± 2.04	5.55 ± 0.48	1.85 ± 0.13	8.09 ± 0.88	15.99 ± 1.48	5.561 ± 5.26
TLR4 + NFKB	1.73 ± 0.14^b	0.94 ± 0.18^b	2.32 ± 0.52^b	1.91 ± 0.10^a	0.92 ± 0.08^a	1.68 ± 1.14^b	1.06 ± 0.10^b	0.98 ± 0.11^b
TLR4 + ERK	5.85 ± 0.54^a	11.60 ± 1.08	14.45 ± 1.48^a	2.29 ± 0.25^a	1.73 ± 0.14	10.94 ± 1.05	15.64 ± 1.41	5.66 ± 0.52
TLR4 + JNK	7.60 ± 0.72^a	11.99 ± 2.03	21.21 ± 2.19	2.11 ± 0.23^a	1.78 ± 0.15	10.88 ± 1.01	15.30 ± 1.49	5.30 ± 0.52
TLR4 + p38	1.68 ± 0.13^b	12.42 ± 1.57	7.12 ± 0.68	2.31 ± 0.21^a	1.78 ± 0.12	11.27 ± 1.12	14.06 ± 1.33	5.75 ± 0.51
TLR4 + PLC	7.34 ± 0.72^a	10.98 ± 1.06	22.88 ± 2.34	5.51 ± 0.59	1.78 ± 0.16	10.68 ± 1.02	14.84 ± 1.41	5.84 ± 0.57
TLR4 + PKC	7.47 ± 0.74^a	10.85 ± 1.09	20.78 ± 2.56	5.30 ± 0.48	1.79 ± 0.13	9.76 ± 0.95	15.71 ± 1.47	5.78 ± 5.57
TLR4 + PI3K	6.99 ± 0.69^a	10.76 ± 1.07	20.06 ± 1.95^a	2.24 ± 0.21^a	1.76 ± 0.16	18.74 ± 1.32^a	28.815 ± 2.85^a	10.670 ± 1.57^a

Variations are expressed as mRNA fold changes using β-actin as an internal standard. Monocytes were first incubated for 30 min with the inhibitors before addition of the ulvan extract at 25 μg/ml for 4 h. Data represents the mean ± SEM of four independent experiments in triplicate. Letters indicate statistically significant differences with the experiment with the TLR inhibitor, ^ap < 0.05, ^bp < 0.01.