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. 2018 Nov 23;12:445. doi: 10.3389/fncel.2018.00445

FIGURE 7.

FIGURE 7

Perturbagen hit pifithrin-μ reduces aminoglycoside-induced hair cell death in cultured mouse utricles. (A) Myosin VIIa- and Hoechst 33342-labeled hair cell counts in the peripheral region of utricles treated with vehicle (0.1% DMSO, gray), pifithrin-μ (10 μM, pink), AT13387 (10 μM, brown), or AEG3482 (25 μM, blue). (B) Hair cell counts labeled as in (A) for the central region of the utricle. Hair cell count results in (A–D) are pooled from two independent experiments with the significant effects replicated in the second experiment (n = 4–12 utricles per group). (C,D) Reducing the concentration of pifithrin-μ to 5 μM reduces the toxicity of the compound but also abolishes the protection against neomycin-induced hair cell death in both the peripheral (C) and central (D) regions of the utricle. Asterisks represent adjusted p-values from Tukey post hoc test results following one-way ANOVA with ∗∗∗ representing p < 0.001, ∗∗∗∗ representing p < 0.0001, and ‘ns’ (not significant) representing p > 0.05. All error bars in (A–D) represent ± SD values.