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. 2018 Sep 21;46(21):11528–11538. doi: 10.1093/nar/gky845

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Purification of CPF and APT complexes. (A) CPF subunits from yeast. Names of proteins used in this work are in bold. Masses were calculated using ProtParam. (B) SDS-PAGE analysis of Pta1-TAPS purification from Streptactin resin, stained with SYPRO Ruby. Subunits were analyzed using tryptic-digest mass spectrometry from excised bands and all 15 previously-known CPF proteins were identified. (C) Purification from a Syc1-tagged yeast strain yields only the seven APT subunits. The gel filtration chromatogram (absorbance at 280 nm, arbitrary units) and corresponding Coomassie-blue stained SDS-PAGE analysis of fractions are shown. An asterisk indicates the tagged subunit.