Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 2;46(21):11618–11626. doi: 10.1093/nar/gky887

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Stacking-induced fluorescence increase of Cy3. (A) Immobile DNA structures designed to probe the possible interactions between the Cy3 dye and the DNA hairpin (see Supplementary Figure S2 for additional structures). (B and C) Time-resolved decays (B) and amplitude-weighted average lifetimes (C) for Cy3 attached to representative duplex structures (the buffer contained 50 mM NaCl). (D) Amplitude-weighted average lifetime as a function of NaCl concentration for hairpins with different dye positions. Moving the dye away from the ssDNA–dsDNA junction reduces the enhancement. (E) AV calculations illustrate that the dye can explore up to four bases along duplex DNA (see Supplementary Figure S7 for expanded versions of these). Error bars in (C) and (D) represent the standard deviation (N = 3).