Figure 5.

Direct interaction between the Set1 N-terminal region and Swd1. (A) Direct binding of purified FLAG-tagged Set1C catalytic core subunits (Swd1, Swd3, Bre2 and Sdc1) (Supplementary Figure S2B) to GST-tagged Set1 fragments (Supplementary Figure S5B) relative to GST. Note that the binding condition in this assays (150 mM salt) was less stringent compared to that of similar experiments in our previous report (300 mM salt) (15). (B) Schematic representations of the Set1 1–229 RRR1, RRR2, RRR1&2 fragments containing amino acids substitution from arginine (underlined) to alanine (bold). (C) Direct binding of purified FLAG-Swd1 to GST-tagged Set1 1–229 WT and mutant fragments (Supplementary Figure S5C). (D) Direct binding of purified FLAG-Swd1 and FLAG-Swd2 to GST-tagged Set1 fragments (Supplementary Figure S5D).