Figure 6.

Requirement of the basic motifs within the Set1 N-terminal region for H3K4 methylation activity of the Spp1-deficient Set1 complex. (A) A schematic diagram of Set1Cs containing Set1 and its derived fragments along with associating subunits. Asterisks indicate arginine to alanine substitutions. Dotted outline and faint depiction for Spp1 indicate its conditional presence in the complexes depending on the inclusion of Spp1 during complex reconstitution. Note that Swd2 and Shg1 are not included for all complex preparations. (B and C) In vitro HMT assays with H2Bub chromatin and indicated purified Set1Cs (Supplementary Figure S6A). Note that all purified complexes used in (B) do not contain Spp1.