Skip to main content
. 2018 Oct 24;46(21):11370–11380. doi: 10.1093/nar/gky991

Figure 1.

Figure 1.

RNA-Best preservation of cultured gonadotropes maintains IEG transcript levels under basal and stimulated conditions. (A, B) RNA yield (A) and quality (B) from fresh cells, RNA-Best-preserved cells, or cells preserved using cryopreservation, methanol fixation, or FRISCR. *P < 0.05 and **P < 0.01; bars show mean ± s.e.m. (C) qPCR analysis of Fos and Egr1 basal expression and GnRH induction (fold change); 2 nM GnRH treatment for 35 min; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Note that Egr1 fold change using FRISCR equals 1, due to no difference between vehicle and GnRH treatment. (D) Time course of GnRH induction of Fos and Egr1 in fresh vs. RNA-Best-preserved cells (n = 6 biological replicates per time point and protocol). Data in A–D are from one of four representative experiments.