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. 2018 Oct 24;46(21):11370–11380. doi: 10.1093/nar/gky991

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — IEGs show bimodal SC responses to GnRH pulse stimulation. (A) Schematic of the time course experiment design. Cells were exposed to four pulses of GnRH (blue arrows) and collected at short time intervals around the 4th pulse (yellow arrows). (B, C) Graphs showing the probability for a SC to express a house-keeping gene (either Eef1a, H2fz, Rps11, or Rps25; (B)) or a regulated gene (as indicated; (C)) following either vehicle or GnRH treatment. Error bars are based on the binomial standard deviation on the number of gene-expressing cells. In C, Right panel, the +25 min and +35 min data points are statistically different from −1 min.