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. 2018 Aug 22;46(21):11202–11213. doi: 10.1093/nar/gky754

Figure 5.

Figure 5.

Knockdown of DYRK1A affects the expression of genes located near its binding sites. (A) Expression analysis of a select group of genes located near the DYRK1A- CBP/p300 occupied regions, in T98G cells infected with lentivirus expressing shRNAs targeting control, DYRK1A, p300, or CBP. (B) Expression analysis of a select group of genes located near DYRK1A-CBP/p300 occupied regions in HEK293 cells, infected with lentivirus expressing shRNA targeting DYRK1A, p300 or CBP. Lentivirus infected cells were selected with 1μg/ml puromycin for 4 days before harvesting (A and B). (C) Expression analysis of genes analysed in (B) in HEK293 cells inducibly expressing Flag-DYRK1A. For induction, cells were treated with 250ng/ml Doxycycline for 40 h. (D) Expression analysis of genes analysed in (B) in HEK293 cells inducibly expressing Flag-DYRK1A-KD. For induction, cells were treated with 250 ng/ml Doxycycline for 40 h. GAPDH mRNA was used to normalize RNA in RT-qPCR samples. Data represent the mean ± SD (n = 3 biological replicates). Student's t test were done to compare samples. P value = * (P < 0.05).