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. 2018 Sep 8;46(21):11251–11261. doi: 10.1093/nar/gky801

Figure 3.

Figure 3.

Effects of Dot1 overexpression and loss of Ubp8 on H2BK123ub1 in chromatin. (A) ChIP-qPCR analysis of H2BK123ub1 relative to H2B in transcribed regions in bre1Δ, WT, ubp8Δ and Dot1-G401A overexpression under the control of the strong TDH3 promoter (mean and individual data points of two biological replicates). (B) Metagene plots of H2BK123ub1 ChIP-seq in WT, ubp8Δ and Dot1-G401A showing the average H2BK123ub1 pattern around the transcription start site. Colored lines represent five different groups based on gene expression level, from high (group 1) to low (group 5) expression. (C) Heatmaps of read-depth normalized H2BK123ub1 ChIP-seq counts showing the H2BK123ub1 signal in the five different gene expression groups indicated in B. Genes within each subgroup were ranked on gene length and centered on the gene midpoint.