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. 2018 Nov 23;9:1710. doi: 10.3389/fpls.2018.01710

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Copyright © 2018 Zang, Geng, He, Wang, Tian, Xin, Yao, Hu, Ni, Sun and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of TaPEPKR2 overexpression on heat tolerance in transgenic Arabidopsis. (A) Real-time PCR of transgenic Arabidopsis lines overexpressing TaPEPKR2. L1–L7 are seven individual TaPEPKR2 transgenic lines. Relative TaPEPKR2 expression in transgenic line L1 was set as the control. Data are the means of three replicates ± SD. (B) The heat treatment regime. (C) Seedlings grown for 5.5 days at 22°C were used as the control. (D) Phenotypes of WT, L1, L3, and L5 after heat treatment and recovery at 22°C for 7 days. (E) Survival rates of three TaPEPKR2 transgenic lines (L1, L3, and L5) and WT after heat treatment. Data are the mean of three replicates ± SD (n = 60 for each experiment). (F) Ion leakage assay of the seedlings in panel (D) at 42°C for 1 h. Data are the means ± SD of three replicates; ^∗∗P < 0.01 (Student’s t-test).