FIGURE 5.

Effects of drought stress on the H₂S-related physiological indices in the mitochondria of WT, des1 and OE-DES1. Leaves of 4-week-old after withholding water for 1 week were collected and the mitochondria were isolated and purified. (A) Cys contents of mitochondria were quantitated by the method described previously (Fu et al., 2012). (B) H₂S contents of mitochondria were measured with electrodes (TBR4100 WPI, Sarasota, FL, United States). (C) H₂S production rates of L-Cys and (D) H₂S production rates of D-Cys in mitochondria of WT, des1 and OE-DES1 were measured with methylene blue method (Fu et al., 2012). Data are means ± SEs of three independent experiments; bars marked with the same letter did not differ significantly at p < 0.05.