Table 1.
Target genes analysed by qPCR
| Gene accession ID | Primer/probe | Sequences (5′–3′) | T m (°C) | Location (bp) | Size (bp) |
|---|---|---|---|---|---|
| IGFALS | F | GCG TCA AGG CCA ATG TCT TC | 59 | 1178–1197 | 63 |
| XM_004021110.1 | R | GGT TGT GGT CCA GGT AGA G | 59 | 1222–1240 | |
| P | 6FAM‐TCA AGC TGC CCA AGC T‐MGBNFQ | 70 | 1199–1214 | ||
| IGF1 | F | CTT CCG GAG CTG TGA TCT GA | 58 | 389–408 | 73 |
| NM_001009774.3 | R | TGA GCG GGC CGA CTT G | 59 | 446–461 | |
| P | 6FAM‐CTG TGC GCC TCT CAA G‐MGBNFQ | 68 | 425–440 | ||
| IGF2 | F | CGA GGC ATC CAG CGA TTA G | 58 | 623–641 | 62 |
| NM_001009311.1 | R | TAG ATG GTG TCA CTT GGC AGA ATT | 59 | 661–684 | |
| P | 6FAM‐AGT GAG CCA AAG TGT C‐MGBNFQ | 69 | 643–658 | ||
| IGF1R | F | TCT AAC TTT GTC TTT GCA AGA ACC A | 58 | 73–97 | 64 |
| AY162434.1 | R | TCA CTG GCC CAG GAA TGT C | 58 | 118–136 | |
| P | 6FAM‐CCT GCA GAA GGA GCA G‐MGBNFQ | 69 | 100–115 | ||
| GHR | F | CAT AAA GCC TGG AGG AAA CCA T | 58 | 119–140 | 59 |
| NM_001009323.2 | R | CCG GCA GAC AAT CCT GAA A | 58 | 159–177 | |
| P | 6FAM‐CGA AAA TCC AGC CTC T‐MGBNFQ | 68 | 142–157 | ||
| IGFBP1 | F | GCC AAA CTG CAA CAA GAA TGG | 59 | 210–221 | 52 |
| NM_001145177.1 | R | TCC GTC CAG CGA AGT CTC A | 59 | 242–261 | |
| P | 6FAM‐TTC TAT CAC AGC AAA CAG T‐MGBNFQ | 69 | 223–241 | ||
| IGFBP3 | F | GCC AGC GCT ACA AGG TTG AC | 59 | 59–78 | 68 |
| EU616622.1 | R | CTT GGA CTC GGA GGA GAA GTT CT | 59 | 104–126 | |
| P | 6FAM‐ACG AGT CTC AGA GCA C‐MGBNFQ | 69 | 80–95 | ||
| SLC2A2 | F | GAA GAA TCA AAG CCC TGT TGG T | 60 | 258–279 | 70 |
| AJ318925.1 | R | TTC GAA AAC CCC ATC AAG AGA | 58 | 307–327 | |
| P | 6FAM‐CAA ACA TTC TTT CAT TAG TTG GA‐MGBNFQ | 69 | 282–304 | ||
| SLC2A4 | F | AAC CCA GCA CAG AAC TGG AGT AC | 58 | 71–93 | 60 |
| AB005283.1 | R | CCT GTG TGG ACC CTC AGT CA | 58 | 110–130 | |
| P | 6FAM‐AGG GCC GGA TGA GA‐MGBNFQ | 68 | 96–109 | ||
| MTOR | F | CTG CAC GTC AGC ACC ATC A | 59 | 3772–3790 | 80 |
| NM_001145455.1 | R | AGC CAT TCC AAC CAA TCA TCT T | 58 | 3830–3851 | |
| P | 6FAM‐CCT CCA AAA GGC C‐MGBNFQ | 69 | 3792–3804 | ||
| NR3C1 | F | GGG CCA ACA TAA TTG GCA ATA A | 60 | 509–531 | 84 |
| NM_001114186.1 | R | CCC AGA GGT ACT CAC ACC ATG A | 59 | 570–592 | |
| P | 6FAM‐ATG TCT GCC ATT TCT‐MGBNFQ | 69 | 532–547 | ||
| RNA18S | Eukaryotic 18S rRNA 6VIC/MGBNFQ | ||||
| ACTB | F | ACC AGT TCG CCA TGG ATG ATG | 61 | 76–96 | 53 |
| NM_001009784.1 | R | CCG GAG CCG TTG TCA AC | 58 | 113–128 | |
| P | 6FAM‐ACG AGC GCA GCA ATA T‐MGBNFQ | 97–112 | |||
| GAPDH | F | GGG CTG CTT TTA ATA CTG GCA AA | 60 | 91–114 | 81 |
| NM_001190390.1 | R | CAT GTA GAC CAT GTA GTG AAG GTC AA | 61 | 146–171 | |
| P | 6FAM‐CAT CGT TGC CAT CAA TG‐MGBNFQ | 120–136 | |||
| HPRT1 | F | AGG TGT TTA TTC CTC ATG GAC TAA TTA TGG | 61 | 1–30 | 75 |
| JN811683.1 | R | CAC CCA TCT CCT TCA TCA CAT CTC | 61 | 52–75 | |
| P | 6FAM‐ACA GGA CCG AAC GAC TG‐MGBNFQ | 31–47 | |||
| PPIA | F | GTA CTG GTG GCA AGT CCA TCT | 60 | 105–125 | 72 |
| JX534530.1 | R | CAG GAC CTG TAT GCT TCA GAA TGA | 60 | 153–176 | |
| P | 6FAM‐ATG GCG AGA AAT TTG‐MGBNFQ | 126–140 | |||
| RPL19 | F | CAA AAA CAA GCG GAT TCT CAT G | 58 | 340–361 | 65 |
| AY158223.1 | R | GCT TCT TGC GAG CCT TGT CT | 58 | 385–404 | |
| P | 6FAM‐AAC ATA TCC ACA AGC TGA A‐MGBNFQ | 363–381 | |||
| YWHAZ | F | GAG GGT CGT CTC CAG TAT TGA G | 60 | 25–46 | 67 |
| NM_001267887.1 | R | TTC TCG AGC CAT CTG CTG TTT T | 61 | 70–91 | |
| P | 6FAM‐CAG CAC CTT CCG TCT TT‐MGBNFQ | 49–65 |
Sequences for forward (F) and reverse (R) primers; fluorescent reporter dye (FAM, VIC) bound TaqMan probes with molecular‐groove binding non‐fluorescence quencher (MGBNFQ). Melting temperature (T m) and base pairs (bp) noted.