Table 2.
Results of two‐way ANOVA comparison between the daily profiles of Per1, Per2, Nr1d1 and Bmal1 expression in the pup liver
| Per1 | Per2 | Nr1d1 | Bmal1 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Liver | F(DFn. DFd) | P | F(DFn. DFd) | P | F(DFn. DFd) | P | F(DFn. DFd) | P | ||
| P10 | Wistar controls vs. cross‐strain | Interaction | F 6,55 = 7.083 | <0.0001 | F 6,54 = 4.636 | 0.0007 | F 6,54 = 4.012 | 0.0022 | F 6,55 = 1.115 | 0.3655 |
| Time | F 6,55 = 7.846 | <0.0001 | F 6,54 = 3.350 | 0.0070 | F 6,54 = 9.820 | <0.0001 | F 6,55 = 10.43 | <0.0001 | ||
| Group | F 1,55 = 115.9 | <0.0001 | F 1,54 = 274.8 | <0.0001 | F 1,54 = 7.621 | 0.0079 | F 1,55 = 2.501 | 0.1195 | ||
| SHR controls vs. cross‐strain | Interaction | F 6,49 = 1.702 | 0.1405 | F 6,49 = 2.246 | 0.0541 | F 6,49 = 0.3672 | 0.8962 | F 6,47 = 3.731 | 0.0041 | |
| Time | F 6,49 = 1.659 | 0.1513 | F 6,49 = 1.912 | 0.0975 | F 6,49 = 4.793 | 0.0006 | F 6,47 = 1.432 | 0.2227 | ||
| Group | F 1,49 = 0.4769 | 0.4931 | F 1,49 = 1.791 | 0.1869 | F 1,49 = 0.0999 | 0.7533 | F 1,47 = 3.272 | 0.0769 | ||
| P30 | Wistar controls vs. cross‐strain | Interaction | F 6,54 = 16.68 | <0.0001 | F 6,54 = 9.325 | 0.0001 | F 6,54 = 12.07 | 0.0001 | F 6,54 = 6.609 | <0.0001 |
| Time | F 6,54 = 25.20 | <0.0001 | F 6,54 = 34.65 | 0.0001 | F 6,54 = 42.71 | <0.0001 | F 6,54 = 33.10 | <0.0001 | ||
| Group | F 1,54 = 35.95 | <0.0001 | F 1,54 = 20.09 | <0.0001 | F 1,54 = 54.16 | <0.0001 | F 1,54 = 101.7 | <0.0001 | ||
| Wistar controls vs. cross‐strain return | Interaction | F 6,56 = 22.80 | <0.0001 | F 6,56 = 4.001 | 0.0021 | F 6,56 = 13.04 | <0.0001 | F 6,56 = 3.657 | 0.0039 | |
| Time | F 6,56 = 25.65 | <0.0001 | F 6,56 = 39.05 | 0.0001 | F 6,56 = 105.0 | <0.0001 | F 6,56 = 31.00 | <0.0001 | ||
| Group | F 1,56 = 11.06 | 0.0016 | F 1,56 = 0.0161 | 0.8994 | F 1,56 = 9.823 | 0.0027 | F 1,56 = 20.00 | <0.0001 | ||
| Wistar controls vs. intra‐strain | Interaction | F 6,53 = 7.124 | <0.0001 | F 6,55 = 4.832 | 0.0005 | F 6,54 = 14.26 | <0.0001 | F 6,55 = 1.739 | 0.1292 | |
| Time | F 6,53 = 8.803 | <0.0001 | F 6,55 = 34.64 | 0.0001 | F 6,54 = 82.71 | <0.0001 | F 6,55 = 17.11 | <0.0001 | ||
| Group | F 1,53 = 0.9994 | 0.3220 | F 1,55 = 1.586 | 0.2132 | F 1,54 = 39.29 | <0.0001 | F 1,55 = 6.884 | 0.0112 | ||
| SHR controls vs. cross‐strain | Interaction | F 6,48 = 5.162 | 0.0004 | F 6,48 = 13.91 | <0.0001 | F 6,48 = 13.02 | <0.0001 | F 6,48 = 11.87 | 0.0001 | |
| Time | F 6,48 = 16.24 | <0.0001 | F 6,48 = 11.81 | <0.0001 | F 6,48 = 26.93 | <0.0001 | F 6,48 = 23.09 | 0.0001 | ||
| Group | F 1,48 = 14.13 | 0.0005 | F 1,48 = 49.75 | <0.0001 | F 1,48 = 16.61 | 0.0002 | F 1,48 = 224.9 | 0.0001 | ||
| SHR controls vs. cross‐strain return | Interaction | F 6,51 = 3.892 | 0.0028 | F 6,51 = 10.43 | <0.0001 | F 6,51 = 0.8747 | 0.5200 | F 6,51 = 1.152 | 0.3465 | |
| Time | F 6,51 = 18.00 | <0.0001 | F 6,51 = 36.87 | <0.0001 | F 6,51 = 15.89 | <0.0001 | F 6,51 = 20.56 | <0.0001 | ||
| Group | F 1,51 = 0.4959 | 0.4845 | F 1,51 = 3.921 | 0.0531 | F 1,51 = 0.5494 | 0.4620 | F 1,51 = 0.0804 | 0.7780 | ||
| SHR controls vs. intra‐strain | Interaction | F 6,50 = 4.443 | 0.0011 | F 6,47 = 7.096 | <0.0001 | F 6,49 = 4.847 | 0.0006 | F 6,48 = 6.770 | <0.0001 | |
| Time | F 6,50 = 22.19 | 0.0001 | F 6,47 = 26.66 | <0.0001 | F 6,49 = 33.60 | 0.0001 | F 6,48 = 31.03 | <0.0001 | ||
| Group | F 1,50 = 13.74 | 0.0005 | F 1,47 = 13.22 | 0.0007 | F 1,49 = 0.0017 | 0.9675 | F 1,48 = 17.18 | 0.0001 | ||
Daily profiles of clock gene expression in the liver of 10‐day‐old (P10) and 30‐day‐old (P30) Wistar rat and SHR pups reared by their own mothers (controls), the foster mother of the different strain (cross‐strain), the foster mother of the same strain (intra‐strain) or they were reared by the foster mother of the different strain from P1 until P10 and then returned to their own mother (cross‐strain return). F test distribution ratio (F), degrees of freedom in the variance of numerator (DFn) and denominator (DFd), P value (P). Significant differences are indicated in bold.