Fig. 4. Electrophoresis of polymerase chain reaction products using primers H1/H2, H3/H4, and FibF/FibR with fragment sizes of (A) 1219 bp, (B) 1319 bp, and (C) 1124 bp amplifying hexon and fiber gene regions of fowl adenovirus (FAdV) in 1% agarose gel from a sample of the original (passage 2 [E2]) and passaged FAdV isolates in specific pathogen-free chicken embryonated eggs (SPF CEE). Lane M, DNA marker 1 kb; Lane 1, positive control (UPM04217); Lane 2, E2 FAdV; Lane 3, E5 FAdV; Lane 4, E10 FAdV; Lane 5, E15 FAdV; Lane 6, E20 FAdV; Lane 7, negative control.
