Fig. 5. Effects of dapsone on the production of tumor necrosis factor alpha (TNF-α) and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-activated bone marrow cells (BMs). (A) BMs were cultured at a concentration of 1 × 106 cells/mL in 96-well culture plates and treated with dapsone (0–100 µg/mL) and/or LPS (1 µg/mL). After 3 days, the culture supernatants were harvested and used for enzyme-linked immunosorbent assay. Data are presented as mean ± SD values. *p < 0.05 and **p < 0.01, respectively, compared to BMs treated with LPS alone (dapsone 0 g/mL). ND, none detectable. (B) BMs were cultured and treated as described in Fig. 2. The cells were harvested after 3 days and stained with dichlorofluorescein diacetate. The numbers in the histograms indicate the geometric mean fluorescence intensity.
