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. 2018 Nov 30;38(6):BSR20180738. doi: 10.1042/BSR20180738

Figure 6. Effects of mutant Akt expression vector on casticin-mediated cell invasion and its related proteins.

Figure 6

MDA-MB-231 and 4T1 cells were respectively transfected with Akt1 cDNA or empty vector, and then treated with or without 0.50 µM of casticin for 24 h. (A,B) The protein expression levels of Akt, p-Akt, and MMP-9 were analyzed by Western blotting and quantitated against the densitometric signal of β-actin bands. *P<0.05 and **P<0.01. (C,D) Cell invasion was analyzed by Boyden chamber assays, and the quantitative data were presented as mean ± S.D. of three independent experiments. *P<0.05 and **P<0.01 compared with the control. ##P<0.01 compared with the untreated MDA-MB-231/Akt1 cDNA group or 4T1/Akt1 cDNA group.