Figure 2. Knockdown of GCN5 inhibited proliferation by IL-6–stimulated in PCa cells.

Cells were cultured and then treated with IL-6 (20 ng/ml) for 24 h, GCN5 siRNAs were then transfected and cultured for another 24 h. (A) The protein expression level of GCN5 was measured using Western blotting assay. (B) Cell proliferation was determined using MTT assay. The error bars represent the means ± S.D. of three independent experiments. *P<0.05 compared with control or NC. #P<0.05 compared with NC.