Figure 6.

Hydrolysis time course measurement of reducing sugar with TKU029 chitosanase. The reaction mixture in a 250 mL Erlenmeyer flask containing 1% water soluble chitosan in 100 mL phosphate buffer (pH 7, 50 mM) and 10 mL of crude enzyme. The hydrolysis condition was carried at 50 °C in 6 h. The hydrolysis solution was then tested reducing sugar under the assay mentioned in the methods section. The error bars represent standard deviations (n = 3).