Figure 3.

Deguelin treatment was not cytotoxic or cytostatic to fibroblasts. (a) NuFF-1 cells were grown to confluence and then treated with vehicle (DMSO) or increasing concentrations of deguelin, as indicated, for 72 h. Cell viability was then assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Data was recorded at 570 nm using a Cytation 3 plate reader. Data is presented as percent (%) inhibition relative to vehicle. (b) NuFF-1 cells were plated at 25% confluence and then treated with vehicle (DMSO) or increasing concentrations of deguelin. Cells were allowed to undergo two cell doublings, and were then assayed using the method used in (a). Data is presented as the number of cells relative to vehicle. Error bars represent the standard deviation of three technical replicates. Replicate assays were performed (n = 3), and a representative biological replicate is shown in each panel. Statistical analyses were performed using Student’s t-test between each indicated concentration relative to the vehicle-treated cells; * p < 0.01.