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. 2018 Nov 7;10(11):614. doi: 10.3390/v10110614

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ganciclovir-resistant HCMV is sensitive to deguelin treatment. NuFF-1 cells were treated with (a) ganciclovir (10 μM) or (b) deguelin (250 nM) for 1 h, after which cultures were infected with either TB40/EmCherry-UL99eGFP (UL99eGFP) or TReGFP for 7 d (moi = 1.0). Vehicle-treated cells in each case received DMSO. Cell-associated viral DNA was assessed by qPCR using primers directed at UL122. Each sample was analyzed in triplicate and normalized to cellular MDM2. Data is presented as the ratio of UL122/MDM2 relative to vehicle. Error bars represent the standard deviation of three technical replicates. Replicate assays were performed (n = 3), and a representative biological replicate is shown in each panel. AU = arbitrary units. Statistical significance was calculated by two-way ANOVA, *** p < 0.0001.