Figure 5.

Treatment of HCMV infected fibroblasts with deguelin decreases IE, E, and L gene transcripts. NuFF-1 cells were treated with vehicle (DMSO) or deguelin (250 nM) for 1 h, after which cultures were infected with UL99eGFP (moi = 1.0). Total RNA was collected at the indicated time points. Viral transcripts were assessed by RTqPCR using the following primers: (a) UL123 and (b) UL122 (IE); (c) UL44 and (d) UL54 (E); (e) UL32 and (f) UL99 (L). Each sample was analyzed in triplicate and normalized to cellular GAPDH. Error bars represent the standard deviation of three technical replicates. Replicate assays were performed (n = 3), and a representative biological replicate is shown in each panel. AU = arbitrary units. Statistical significance was calculated by two-way ANOVA, * p < 0.05, ** p < 0.005, *** p < 0.0001.